使用muscle进行了多序列比对,但是想要建树时,总是提示数据不正确,如图所示:
使用iqtree建树时会提示:
iqtree -s text.phy -m MFP -b 1000 -nt AUTO(原命令)
Reading alignment file text.phy ... Phylip format detected
ERROR: Sequence pfu____aug2.0 contains not enough characters (1680)
ERROR: Duplicated sequence name Cluster-17
使用phyml建树时会提示:
PhyML -i text.phy -d aa -b 1000 -m LG -f m -v e -a e -o tlr(原命令)
. Err: Symbol "." should not appear in the first sequence
. Type enter to exit.
. One or more missing sequences in block 1.
. Type enter to exit.
我尝试对用Mafft进行多序列比对,但是得到的fasta数据不知该如何转换成phylip格式。现在卡在了对数据的处理阶段,急需帮助!感谢!