velocyto 运行出现“WARNING - Not found cell and umi barcode in entry 1003 of the bam file”
以下是我运行记录:
2024-08-08 13:12:20,559 - ERROR - This is an older version of cellranger, cannot check if the output are ready, make sure of this yourself
2024-08-08 13:12:20,560 - DEBUG - Using logic: Default
2024-08-08 13:12:20,580 - INFO - Read 6465 cell barcodes from /home/data/t100554/GSE130973/re6/outs/filtered_feature_bc_matrix/barcodes.tsv.gz
2024-08-08 13:12:20,580 - DEBUG - Example of barcode: AAACCTGAGGCCGAAT and cell_id: re6:AAACCTGAGGCCGAAT-1
2024-08-08 13:12:20,582 - DEBUG - Peeking into /home/data/t100554/GSE130973/re6/outs/possorted_genome_bam.bam
2024-08-08 13:12:20,697 - WARNING - Not found cell and umi barcode in entry 0 of the bam file
2024-08-08 13:12:20,698 - WARNING - Not found cell and umi barcode in entry 111 of the bam file
2024-08-08 13:12:20,698 - WARNING - Not found cell and umi barcode in entry 256 of the bam file
2024-08-08 13:12:20,699 - WARNING - Not found cell and umi barcode in entry 872 of the bam file
2024-08-08 13:12:20,700 - WARNING - Not found cell and umi barcode in entry 974 of the bam file
2024-08-08 13:12:20,700 - WARNING - Not found cell and umi barcode in entry 978 of the bam file
2024-08-08 13:12:20,703 - WARNING - The file /home/data/t100554/GSE130973/re6/outs/cellsorted_possorted_genome_bam.bam already exists. The sorting step will be skippe
2024-08-08 13:12:20,703 - INFO - Load the annotation from /home/data/t100554/refdata-gex-GRCh38-2024-A/genes/genes.gtf
2024-08-08 13:12:26,722 - DEBUG - Parsing Chromosome GL000009.2 strand - [line 0]
2024-08-08 13:12:26,722 - DEBUG - Done with GL000009.2- [line 7]
2024-08-08 13:12:26,722 - DEBUG - Assigning indexes to genes
2024-08-08 13:12:26,722 - DEBUG - Seen 1 genes until now
2024-08-08 13:12:26,722 - DEBUG - Parsing Chromosome GL000194.1 strand - [line 8]
2024-08-08 13:12:26,722 - DEBUG - Done with GL000194.1- [line 33]
2024-08-08 13:12:26,722 - DEBUG - Assigning indexes to genes
2024-08-08 13:12:26,722 - DEBUG - Seen 3 genes until now
2024-08-08 13:12:26,722 - DEBUG - Parsing Chromosome GL000195.1 strand - [line 34]
2024-08-08 13:12:26,722 - DEBUG - Done with GL000195.1- [line 43]
......
后续继续运行中,并未出现错误而停止
关于 warning这几行我尝试根据这篇贴文进行解决https://cloud.tencent.com/developer/article/1877797
但还出现这个问题。请问我需要在意这几行waring嘛?
还有关于第一行的“ERROR - This is an older version of cellranger, cannot check if the output are ready, make sure of this yourself”,我安装的是最新版,为什么还会这样子呢?但没有影响后续的运行,这个要如何解决呢?
感谢大家解答我的疑惑!!