FindAllMarkers时报错数据层未合并，seurat如何合并？

Question

scobj.markers<-FindAllMarkers(scobj,

•                            only.pos=TRUE,
  

•                          min.pct=0.25)
  

  Calculating cluster 0
  Calculating cluster 1
  警告: No DE genes identified
  警告: The following tests were not performed:
  警告: When testing 0 versus all:
  data layers are not joined. Please run JoinLayers
  警告: When testing 1 versus all:
  data layers are not joined. Please run JoinLayers
  警告: When testing 2 versus all:
  data layers are not joined. Please run JoinLayers